Upregulated MiR-1269 in hepatocellular carcinoma and its clinical significance

作者：Gan, Ting-Qing; Tang, Rui-Xue; He, Rong-Quan; Dang, Yi-Wu; Xie, You; Chen, Gang^*

来源：International Journal of Clinical and Experimental Medicine, 2015, 8(1): 714-721.

• miR-1269
• hepatocellular carcinoma
• paraffin-embedded tissues
• RT-qPCR
• target prediction

Objectives: MicroRNAs (miRNAs) are small, non-coding RNAs that have been increasingly shown important roles in various classes of cancers. However, miR-1269 has not been comprehensively studied in hepatocellular carcinoma (HCC). Thus, the purpose of the study was to evaluate the relationship between the expression of miR-1269 and clinicopathological parameters in HCC patients, and to predict its potential target genes. Methods: Total RNA was extracted from 95 pairs of HCC and matching adjacent non-cancerous tissues. The level of miR-1269 expression was detected by using quantitative real-time RT-PCR and calculated with the 2(-Delta Cq) method. Eighteen online biological databases were used for targets prediction. Results: MiR-1269 expression was up-regulated in HCC tissues (1.9264 +/- 0.7160) compared to their non-tumor livers (1.5518 +/- 0.7273, P < 0.001). Level of miR-1269 was positively correlated to tumor nodes (r = 0.206, P = 0.046), metastasis (r = 0.203, P = 0.049), portal vein tumor embolus (r = 0.247, P = 0.016), vaso-invasion (r = 0.273, P = 0.008), tumor capsular infiltration (r = 0.407, P < 0.001) and expression of MTDH (r = 0.211, P = 0.005). Finally, 7 databases could be applied for the target prediction successfully. There were 9 targeted genes which had been shown concurrently by at least 4 databases: AGAP1, AGK, BPTF, C16orf74, DACT1, LIX1L, RBMS3, ZNF706 and BMPER. Conclusions: MiR-1269 may be possibly involved in the tumorigenesis and progress of HCC. MiR-1269 could also act as a potential biomarker for the prognosis prediction for HCC.

• 出版日期2015
• 单位广西医科大学