Ultrasonication extraction and gel permeation chromatography clean-up for the determination of polycyclic aromatic hydrocarbons in edible oil by an isotope dilution gas chromatography-mass spectrometry

作者：Wang, Jian-Hua^*; Guo, Cui

来源：Journal of Chromatography A, 2010, 1217(28): 4732-4737.

DOI：10.1016/j.chroma.2010.05.008

• GPC
• Polycyclic aromatic hydrocarbons
• Edible oil
• Gas chromatography-mass spectrometry
• Isotope dilution approach

An analytical method for the determination of US EPA priority pollutant 16 polycyclic aromatic hydrocarbons (PAHs) in edible oil was developed by an isotope dilution gas chromatography-mass spectrometry (GC-MS). Extraction was performed with ultrasonication mode using acetonitrile as solvent, and subsequent clean-up was applied using narrow gel permeation chromatographic column. Three deuterated PAHs surrogate standards were used as internal standards for quantification and analytical quality control. The limits of quantification (LOQs) were globally below 0.5 ng/g, the recoveries were in the range of 81-96%, and the relative standard deviations (RSDs) were lower than 20%. Further trueness assessment of the method was also verified through participation in international cocoa butter proficiency test (T0638) organised by the PAPAS with excellent results in 2008. The results obtained with the described method were satisfying (z <= 2). The method has been applied to determine PAH in real edible oil samples.

• 出版日期2010-7-9
• 单位中华人民共和国山东出入境检验检疫局; 青岛大学