Anti-proliferative effect of the extract of Guangzao (Fructus Choerospondiatis) on cultured rat cardiac fibroblasts

作者：Jia Lei; Yang Yumin; Zhu Deli; Gao Xiaohui; Wang Xiaoling; Xing Huihui; Bao Junping; Shu Liang^*

来源：Journal of Traditional Chinese Medicine, 2015, 35(6): 685-689.

DOI：10.1016/s0254-6272(15)30160-6

• Flavones
• Guangzao (Fructus Choerospondiatis)
• Angiotensin II
• Fibroblasts
• Nitric oxide
• Cyclic GMP

OBJECTIVE: To ascertain if total flavonoids of Guangzao (Fructus Choerospondiatis) (TFFC) extracted from Guangzao (Fructus Choerospondiatis) can inhibit angiotensin II-induced proliferation of cardiac fibroblasts (CFs).
METHODS: CFs were cultured by the differential attachment method. A model of cell proliferation was established by stimulation with Ang II. Cardiac fibroblasts growth was determined using a hemocytometer. Cell proliferation was detected by methyl thiazole tetrazolium. Lactate dehydrogenase activity-was measured by chemical colorimetric method.
RESULTS: Proliferation of TFFC-treated (25, 50, 100 mg/L) fibroblasts was significantly less than that of cells in the angiotensin II group (P < 0.01), and TFFC inhibited proliferation in a dose-dependent manner. These inhibitory effects were partly blocked by pretreatment with NG-nitro-L-arginine methyl ester (L-NAME) and 1H-[1,2,4]-oxadiazole-[4,3-a]-quinoxalin-1-one (ODQ).
CONCLUSION: TFFC inhibited angiotensin II-induced proliferation of cardiac fibroblasts via a mechanism that probably involves activation of the NO-cyclic guanosine monophosphate signaling pathway.

• 出版日期2015-12-15
• 单位内蒙古医科大学; 内蒙古自治区人民医院