Altered expression of caveolin 2 and 3 in smooth muscle of rat urinary bladder by 17 beta-estradiol

作者：Kim Sun Ouck; Song Seung Hee; Lee Seung Chul; Cho Kyung Aa; Park Jong Sung; Kwon Dongdeuk^*; Park Kwangsung

来源：BMC Urology, 2013, 13(1): 44.

DOI：10.1186/1471-2490-13-44

• Caveolin
• Bladder
• Estrogen
• Rat

Background: The purpose of this study was to investigate the effect of estrogen alteration on the expression of caveolin 2 and 3 in rat smooth muscle of urinary bladder. %26lt;br%26gt;Methods: Female Sprague-Dawley rats were divided into three groups: control, bilateral ovariectomy (Ovx), and bilateral ovariectomy followed by subcutaneous injections of 17 beta-estradiol (Ovx + Est). After 4 weeks, urodynamic measurements were taken to ascertain the contraction interval and contraction pressure. The expression and cellular localization of caveolin 2 and 3 were determined by Western blot and immunohistochemistry in rat urinary bladder smooth muscle. %26lt;br%26gt;Results: In cystometrograms, the contraction interval (min) was significantly lower in the Ovx group (3.1 +/- 1.5) than in the control group (5.6 +/- 1.2), but was increased after estrogen treatment (9.3 +/- 1.0). Conversely, the average contraction pressure (mmHg) was higher in the Ovx group (26.2 +/- 2.3) than in the control group (21.9 +/- 3.1), and was decreased after estrogen treatment (23.8 +/- 3.5). Caveolin 2 and 3 expression was localized in the cell membrane of the smooth muscle. The protein expression of both caveolin 2 and 3 was significantly lower after ovariectomy and was restored to the control levels after 17 beta-estradiol treatment. %26lt;br%26gt;Conclusions: Hormonal alteration causes a significant change in the expression of caveolin 2 and 3 in smooth muscle of rat urinary bladder. These findings suggest that these molecules might have functional roles in the detrusor overactivity that occurs in association with hormonal alteration.

• 出版日期2013-9-16
• 单位河北医科大学