Molecular detection of a novel paramyxovirus in fruit bats from Indonesia

作者：Sasaki Michihito; Setiyono Agus; Handharyani Ekowati; Rahmadani Ibenu; Taha Siswatiana; Adiani Sri; Subangkit Mawar; Sawa Hirofumi; Nakamura Ichiro; Kimura Takashi^*

来源：Virology Journal, 2012, 9: 240.

DOI：10.1186/1743-422X-9-240

Background: Fruit bats are known to harbor zoonotic paramyxoviruses including Nipah, Hendra, and Menangle viruses. The aim of this study was to detect the presence of paramyxovirus RNA in fruit bats from Indonesia. %26lt;br%26gt;Methods: RNA samples were obtained from the spleens of 110 fruit bats collected from four locations in Indonesia. All samples were screened by semi-nested broad spectrum reverse transcription PCR targeting the paramyxovirus polymerase (L) genes. %26lt;br%26gt;Results: Semi-nested reverse transcription PCR detected five previously unidentified paramyxoviruses from six fruit bats. Phylogenetic analysis showed that these virus sequences were related to henipavirus or rubulavirus. %26lt;br%26gt;Conclusions: This study indicates the presence of novel paramyxoviruses among fruit bat populations in Indonesia.

• 出版日期2012-10-19