Sox9 Is Upstream of MicroRNA-140 in Cartilage

作者：Nakamura Yukio; He Xinjun; Kato Hiroyuki; Wakitani Shigeyuki; Kobayashi Tatsuya; Watanabe Sumiko; Iida Atsumi; Tahara Hideaki; Warman Matthew L; Watanapokasin Ramida^*; Postlethwait John H

来源：Applied Biochemistry and Biotechnology, 2012, 166(1): 64-71.

DOI：10.1007/s12010-011-9404-y

• MicroRNA-140 (miR-140)
• Sox9
• Cartilage
• Zebrafish
• T/C-28
• siRNA
• In situ hybridization
• RT-PCR

MicroRNA-140 (miR-140) is specifically expressed in developing cartilage tissues. We have previously reported that miR-140 plays an important role during palatal cartilage development by modulating platelet-derived growth factor receptor alpha (pdgfra) in zebrafish. However, the regulatory mechanism of miR-140 in cartilage is still unknown. Using developing zebrafish, sox9a mutant (sox9a-/-) and sox9b mutant (sox9b-/-) zebrafish and SOX9 small interfering RNA in human chondrocytes, T/C-28 cells, we found that miR-140 is regulated by the cartilage master transcription regulator Sox9 in zebrafish and mammalian cells.

• 出版日期2012-1
• 单位河北医科大学