Prolyl Isomerase Pin1 Protects Mice from Endotoxin Shock

作者：Akiyama Hirotada^*; Misawa Takuma; Ono Masao; Uchida Chiyoko; Uchida Takafumi

来源：PLos One, 2011, 6(2): e14656.

DOI：10.1371/journal.pone.0014656

Background: Prolyl isomerase Pin1 may be involved in innate immunity against microbial infection, but the mechanism how Pin1 controls the innate immunity is poorly understood.
Methodology/Principal Findings: Injection of lipopolysaccharide (LPS) into the mice induces inflammatory pulmonary disorder and sometimes the serious damages lead to death. Comparing to the wild-type (WT) mice, the Pin1(-/-) mice showed more serious damages in lung and the lower survival rate after the LPS injection. We compared the levels of typical inflammatory cytokines. Pin1(-/-) mice overreacted to the LPS injection to produce inflammatory cytokines, especially IL-6 more than WT mice. We showed that Pin1 binds phosphorylated PU. 1 and they localize together in a nucleus. These results suggest that Pin1 controls the transcriptional activity of PU. 1 and suppresses overreaction of macrophage that causes serious damages in lung.
Conclusions/Significance: Pin1 may protect the mice from serious inflammation by LPS injection by attenuating the increase of IL-6 transcription of the mouse macrophages.

• 出版日期2011-2-4