Alteration in the expression of exon IIC transcripts of brain-derived neurotrophic factor gene by simvastain in chronic mild stress in mice: a possible link with dopaminergic pathway

作者：Rana Digvijay G; Patel Amrutlal K; Joshi Chaitanya G; Jhala Mayurdhvaj K; Goyal Ramesh K^*

来源：Canadian Journal of Physiology and Pharmacology, 2014, 92(12): 985-992.

DOI：10.1139/cjpp-2014-0125

• BDNF exon II transcripts
• cholesterol
• depression
• dopamine
• statins

We have investigated the influence of dopaminergic agents on the expression of brain-derived neurotrophic factor (BDNF) gene in relation with lipid levels in chronic mild stress (CMS). Mice subjected to CMS were treated with simvastatin (10 mg/kg, per os (orally)) along with bromocriptine (2 mg/kg, intraperitoneally (ip)), levodopa (200 mg/kg, ip), or haloperidol (0.1 mg/kg, ip) for 14 days. CMS produced a decrease in sucrose intake and an increase in serum cholesterol and triglycerides levels with a decrease in high-density lipoprotein cholesterol, which were prevented by simvastatin. This was greater when it was combined with bromocriptine or levodopa. Haloperidol significantly prevented the simvastatin-induced increase in sucrose intake but not the alterations in lipids. There was an upregulation in the expression of BDNF exon-IIA and -IIB transcripts by CMS but not the exon-IIC transcripts. Simvastatin could increase expression of exon-IIC transcripts in stressed mice. This was partially increased by bromocriptine. Haloperidol significantly prevented simvastatin-induced increase in expression of BDNF exon-IIC transcripts. The results showed a positive correlation between expression of BDNF exon-IIC transcripts and sucrose intake. In conclusion, our data suggest the involvement of lipid levels and BDNF exon-IIC transcripts in CMS-induced behaviour in mice, possibly through the dopaminergic system.

• 出版日期2014-12