Molecular epidemiology of rotaviruses among healthy calves in Japan: Isolation of a novel bovine rotavirus bearing new P and G genotypes

作者：Abe Masako; Ito Naoto; Morikawa Shigeki; Takasu Masaki; Murase Tetsuma; Kawashima Takanori; Kawai Yoshihiro; Kohara Junko; Sugiyama Makoto^*

来源：Virus Research, 2009, 144(1-2): 250-257.

DOI：10.1016/j.virusres.2009.05.005

• Bovine rotavirus
• G genotype
• P genotype
• Novel genotype
• Asymptomatic infection
• Japan

A total of 171 fecal specimens collected from healthy calves on a beef farm in Gifu Prefecture, Japan in 2006-2007 were examined for group A rotaviruses by RT-semi-nested PCR targeting the coding region for VP8*. Nine specimens were positive for rotavirus. G and P genotyping indicated that one strain was G10P[11]-like and six strains were considered to be the same unknown G and P genotypes. Among these six untypeable strains, one strain, AzuK-1, was adapted to cell culture and analyzed. Sequence and phylogenetic analyses of the full lengths of VP4 and VP7 genes revealed that AzuK-1 strain is a novel bovine rotavirus bearing new G21 and P[29] genotypes as confirmed by the RCWG. Furthermore, we detected G21P[29] rotaviruses in fecal specimens collected from healthy calves in Hokkaido, Japan during the period from 1997 to 1998. These findings suggest that novel G21P[29] rotaviruses have been widely prevalent among cattle for over 10 years in Japan.

• 出版日期2009-9