Diacetyl and 2,3-pentanedione exposure of human cultured airway epithelial cells: Ion transport effects and metabolism of butter flavoring agents

作者：Zaccone Eric J; Goldsmith W Travis; Shimko Michael J; Wells J R; Schwegler Berry Diane; Willard Patsy A; Case Shannon L; Thompson Janet A; Fedan Jeffrey S^*

来源：Toxicology and Applied Pharmacology, 2015, 289(3): 542-549.

DOI：10.1016/j.taap.2015.10.004

• Diacetyl
• 2,3-Pentanedione
• Ion transport
• Human airway epithelial cells
• Vapor exposure
• Dicarbonyl/L-xylulose reductase

Inhalation of butter flavoring by workers in the microwave popcorn industry may result in "popcorn workers lung." In previous in vivo studies rats exposed for 6 h to vapor from the flavoring agents, diacetyl and 2,3-pentanedione, acquired flavoring concentration-dependent damage of the upper airway epithelium and airway hyporeactivity to inhaled methacholine. Because ion transport is essential for lung fluid balance, we hypothesized that alterations in ion transport may be an early manifestation of butter flavoring-induced toxicity. We developed a system to expose cultured human bronchial/tracheal epithelial cells (NHBEs) to flavoring vapors. NHBEs were exposed for 6 h to diacetyl or 2,3-pentanedione vapors (25 or >= 60 ppm) and the effects on short circuit current and transepithelial resistance (R-t) were measured. Immediately after exposure to 25 ppm both flavorings reduced Na+ transport, without affecting Cl- transport or Na+, K(-)pump activity. R-t was unaffected. Na+ transport recovered 18 h after exposure. Concentrations (100-360 ppm) of diacetyl and 2,3-pentanedione reported earlier to give rise in vivo to epithelial damage, and 60 ppm, caused death of NHBEs 0 h post-exposure. Analysis of the basolateral medium indicated that NHBEs metabolize diacetyl and 2,3-pentanedione to acetoin and 2-hydroxy3-pentanone, respectively. The results indicate that ion transport is inhibited transiently in airway epithelial cells by lower concentrations of the flavorings than those that result in morphological changes of the cells in vivo or in vitro. Published by Elsevier Inc.

• 出版日期2015-12-15