Decreased length of telomeric DNA sequences and increased numerical chromosome aberrations in human osteoarthritic chondrocytes

作者：Tamayo Maria; Mosquera Alejandro; Rego Ignacio; Blanco Francisco J; Gosalvez Jaime; Luis Fernandez Jose

来源：Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 2011, 708(1-2): 50-58.

DOI：10.1016/j.mrfmmm.2011.01.007

• Osteoarthritis
• Chondrocytes
• Aging
• Chromosomes
• Telomeres
• Aneuploidy

Length of telomeric DNA sequences and numerical chromosome aberrations from uncultured human osteoarthritic (OA) articular chondrocytes were compared with those from peripheral blood leukocytes (PBL) from the same individual and from chondrocytes and PBL from control subjects. Cells were both obtained from 39 OA patients (age range: 43-80 years) and from 20 control subjects (age range: 39-94 years). Mean length of telomeric DNA sequences was determined using a quantitative real-time polymerase chain reaction (qPCR) assay and numerical chromosome aberrations were identified in interphase nuclei by Fluorescence In Situ Hybridization (FISH) using cocktails of specific DNA probes for chromosomes 7, 8 and for 18, X and Y. Chondrocytes revealed higher telomere size than PBL, both in control subjects and in OA patients, being 2 and 1.6 times higher respectively, thus revealing cell type specific differences. However, chondrocytes from OA patients showed significantly shorter telomere size than chondrocytes from control subjects (T/S ratio 1.64 +/- 0.41 vs. 1.99 +/- 0.54; mean +/- sd; p = 0.008). Regarding the percentage of numerical chromosome aberrations, OA chondrocytes showed 1.7 times higher than chondrocytes from control subjects (19.80 +/- 3.31 vs. 11.48 +/- 4.11; p < 0.01) and 1.5 times average higher than that from PBL from the own OA patient (13.06 +/- 1.45; p < 0.001). Moreover, PBL from OA patients also showed 1.4 times more anomalies than PBL from controls (13.06 +/- 1.45 vs. 9.54 +/- 1.61; p < 0.001). No significant differences were found between chondrocytes and PBL in control subjects. Chromosome loss was the more frequent aneuploidy, mainly monosomy 18. The decreased telomere size and increased chromosome instability in chondrocytes from OA affected joints may imply a local advanced senescence that could contribute to the pathogenesis or progression of the degenerative articular disease. Moreover, the increased chromosomal abnormalities in PBL from OA patients suggest a more general accelerated senescence phenotype that could promote the age-related degenerative joint pathology.

• 出版日期2011-3-15