科研之友
微信
新浪微博
Facebook
分享链接
摘要
The proliferation of cardiac fibroblasts (CFs) and excessive deposition of extracellular matrix (ECM) are the main pathological characteristics of cardiac fibrosis. In recent years, microRNAs (miRNAs) have been found to be a new kind of regulator in cardiac fibrosis. The purpose of this study was to investigate the role of microRNA-9 (miR-9) in the process of cardiac fibrosis and its mechanism. Treatment of cultured neonatal rat CFs with PDGF-BB or serum suppressed the expression of miR-9. Overexpression of miR-9 obviously inhibited neonatal rat CFs proliferation and collagen production as detected by MTT assays, qRT-PCR, and western blotting. The effects of miR-9 in CFs were abrogated by co-transfection with miR-9 inhibitors. Overexpression of miR-9 reduced the mRNA and protein levels of PDGFR-beta and its downstream protein, extracellular signal-regulated kinase (ERK) 1/2. Silencing PDGFR-beta by small interfering RNA mimicked the anti-fibrotic action of miR-9, whereas overexpression of PGDFR-beta canceled the effect of miR-9 in cultured CFs. Dual-luciferase reporter assays showed that PDGFR-beta was a direct target of miR-9. Overexpression of miR-9 inhibited cardiac fibrosis by targeting PDGFR-beta, indicating that miR-9 might play a role in the treatment of cardiac fibrosis.
