Ability of a Lactobacillus rhamnosus strain cultured in milk whey based medium to bind aflatoxin B-1

作者：Bovo Fernanda; Franco Larissa Tuanny; Rosim Roice Eliana; Fernandes de Oliveira Carlos Augusto

来源：Food Science and Technology, 2014, 34(3): 566-570.

DOI：10.1590/1678-457X.6373

• aflatoxin B-1
• decontamination
• lactic acid bacteria
• milk whey
• MRS broth

This study aimed to compare Lactobacillus rhamnosus growth in MRS (de Man, Rogosa and Sharpe) broth and a culture medium containing milk whey (MMW) and to evaluate aflatoxin B-1 (AFB(1)) adsorption capacity by bacterial cells produced in both culture media. L. rhamnosus cells were cultivated in MRS broth and MMW (37 degrees C, 24 hours), and bacterial cell concentration was determined spectrophotometrically at 600 nm. AFB(1) (1 mu g/ml) adsorption assays were conducted using 1 x 10(10) non-viable L. rhamnosus cells (121 degrees C, 15 minutes) at pHs 3.0 and 6.0 and contact time of 60 minutes. AFB(1) quantification was performed by High Performance Liquid Chromatography. Bacterial cell concentration in MMW was higher (9.84 log CFU/ml) than that in MRS broth (9.63 log CFU/ml). There were no significant differences between AFB(1) binding results at the same pH value (3.0 or 6.0) for the cells cultivated in MRS broth (46.0% and 35.8%, respectively) and in MMW (43.7% and 25.8%, respectively), showing that MMW can adequately replace the MRS broth. Therefore, it can be concluded that the use of L. rhamnosus cells cultivated in MMW offers advantages such as reduction in large scale production costs, improvement of environmental sustainability, and being a practicable alternative for decontamination of food products susceptible to aflatoxin contamination.

• 出版日期2014-9
• 单位中国林业科学研究院