Using surface-enhanced Raman spectroscopy to probe for genetic markers on single-stranded DNA

作者：Moody Benjamin^*; Leotaud John; McCarty Gregory S

来源：Journal of Biomedical Optics, 2010, 15(2): 027014.

DOI：10.1117/1.3400702

• genetic markers
• Raman spectroscopy
• polymorphisms
• DNA

Methods capable of quickly and inexpensively collecting genetic information are of increasing importance. We report a method of using surface-enhanced Raman spectroscopy to probe single-stranded DNA for genetic markers. This unique approach is used to analyze unmodified genes of moderate length for genetic markers by hybridizing native test oligonucleotides into a surface-enhanced Raman complex, vastly increasing detection sensitivity as compared to traditional Raman spectroscopy. The Raman complex is formed by sandwiching the test DNA between 40-nm gold nanoparticles and a photolithographically defined gold surface. With this design, we are able to collect characteristic Raman spectra about the test DNA and to detect genetic markers such as single-nucleotide polymorphisms (SNPs) and polymorphic regions. Results show that strands containing one of three different types of polymorphism can be differentiated using statistically significant trends regarding Raman intensity.

• 出版日期2010-4