Stem cell labeling with iron oxide nanoparticles: impact of 3D culture on cell labeling maintenance

作者：Kilian Teresa; Fidler Florian; Kasten Annika; Nietzer Sarah; Landgraf Veronika; Weiss Katrin; Walles Heike; Westphal Fritz; Hackenberg Stephan; Gruettner Cordula; Steinke Maria^*

来源：Nanomedicine, 2016, 11(15): 1957-1970.

DOI：10.2217/nnm-2016-0042

• metal nanoparticles
• regenerative medicine
• stem cells

Aim: We aimed to analyze the suitability of nanoparticles (M4E) for safe human mesenchymal stem cell (hMSC) labeling and determined cell labeling maintenance in 2D and 3D culture. Materials & methods: We investigated cell-particle interaction and the particles' impact on cell viability, growth and proliferation. We analyzed cell labeling maintenance in 2D and 3D culture invasively and noninvasively. Results: M4E do not affect cell viability, growth and proliferation and do not cause chromosomal aberrations. Cell labeling maintenance is up to five-times higher in 3D conditions compared with 2D culture. Conclusion: M4E allow safe hMSC labeling and noninvasive identification. Our hMSC-loaded, 3D tissue-engineered construct could serve as a graft for regenerative therapies, in which M4E-labeled hMSCs can migrate to their target.

• 出版日期2016-8