Activation of AMPK by berberine promotes adiponectin multimerization in 3T3-L1 adipocytes

作者：Li, Yun; Wang, Pengcheng; Zhuang, Yuan; Lin, Huan; Li, Yehua; Liu, Ling; Meng, Qinghang; Cui, Ting; Liu, Jing; Li, Zhen^*

来源：FEBS LETTERS, 2011, 585(12): 1735-1740.

DOI：10.1016/j.febslet.2011.04.051

• Adiponectin
• Multimerization
• AMPK
• Berberine
• Insulin sensitivity

Adiponectin is assembled into trimer (LMW), hexamer (MMW) and high-molecular-weight (HMW) multimer in adipocytes. The HMW adiponectin is more metabolically active and closely associated with peripheral insulin sensitivity. In this study, we reported that berberine, an isoquinoline alkaloid with insulin-sensitizing effect, inhibits the expression of adiponectin, but promotes the assembly of HMW adiponectin and increases the ratio of HMW to total adiponectin. Berberine activates AMPK. Knockdown of AMPK alpha 1 abolishes the effect of berberine. Activation of AMPK by AICAR also increases the level of HMW adiponectin. Our study suggested that activation of AMPK by berberine promotes adiponectin multimerization. @@@ Structured summary of protein interactions: @@@ Adiponectin physically interacts with Adiponectin by comigration in gel electrophoresis (View Interaction 1, 2) @@@ Adiponectin physica

• 出版日期2011-6-23
• 单位清华大学