Dimeric inhibitors of human saflvary alpha-amylase from emmer (Triticum dicoccon Schrank) seeds

作者：Fontanini Debora^*; Capocchi Antonella; Muccilli Vera; Saviozzi Franco; Cunsolo Vincenzo; Saletti Rosaria; Foti Salvatore; Galleschi Luciano

来源：Journal of Agricultural and Food Chemistry, 2007, 55(25): 10452-10460.

DOI：10.1021/jf071739w

• human salivary alpha-amylase
• dimeric inhibitor
• emmer
• tandem mass spectrometry

The proteins belonging to the cereal trypsin/alpha-amylase inhibitor family are abundant water/salt-soluble flour proteins active against alpha-amylases from several seed parasites and pests and inactive against enclogenous alpha-amylases. Three alpha-amylase inhibitor families have been described in cereals that vary in size and are differently expressed among Triticeae seeds. The present work investigates the presence of human salivary alpha-amylase inhibitors in emmer (Triticum dicoccon Schrank) flour. The isolation was obtained by a series of chromatography steps, and the purification progress was monitored through the inhibition of human salivary alpha-amylase activity. The purified fraction was subjected to protein sequencing by tandem mass spectrometry (MSMS) of the tryptic digests obtained after the sample separation on 2-DE. MSMS data indicated that the emmer alpha-amylase inhibitory fraction was composed of two newly identified proteins [emmer dimeric inhibitor 1 (EDI-1) and emmer dimeric inhibitor 2 (EDI-2)] sharing very high identity levels with related proteins from Triticum aestivum.

• 出版日期2007-12-12