Activation of Avian Aryl Hydrocarbon Receptor and Inter-species Sensitivity Variations by Polychlorinated Diphenylsulfides

作者：Zhang, Rui; Zhang, Xiaowei^*; Zhang, Junjiang; Qu, Ruijuan; Zhang, Jiamin; Liu, Xing; Chen, Jun; Wang, Zunyao; Yu, Hongxia

来源：Environmental Science & Technology, 2014, 48(18): 10948-10956.

DOI：10.1021/es502641f

• CORMORANT PHALACROCORAX-CARBO
• CYTOCHROME-P4501A INDUCTION
• HEPATOCYTE CULTURES
• DIOXIN
• SULFIDES
• POTENCY
• CHICKEN
• PREDICT
• MECHANISMS
• DIVERSITY

It was hypothesized that polychlorinated diphenyl sulfides (PCDPSs) can potentially interact with an aryl hydrocarbon receptor (AHR) and thereby cause adverse effects in wildlife like birds. A recently developed avian AHR1-luciferase report gene (LRG) assay was used to assess the interaction between avian AHR1 and 18 PCDPSs and to compare the interspecies sensitivity among chicken, ring-necked pheasant, and Japanese quail by PCDPSs. Most of the tested PCDPSs could activate the AHR1-mediated pathways in avian species, and the relative potency (ReP) of the PCDPSs increased with the increasing number of substituted Cl atoms. The rank orders of PCDPSs potency were generally similar among birds, although the ReP varied. In addition, not all the sensitivity rank orders of avian AHR1 constructs for PCDPSs were consistent with that of TCDD. ReP values of PCDPSs suggested that some PCDPSs like 2,3,3',4,5,6-hexa-CDPS and 2,2',3,3',4,5,6-hepta-CDPS are higher than the avian WHO-TEFs of OctaCDD, OctaCDF, and most of the coplanar PCBs. Our results report for the first time the activation of an AHR1-mediated molecular toxicological mechanism by PCDPSs, and provide the ranking of ReP and relative sensitivity values of different congeners, which could guide the further toxicity test of this group of potential high priority environmental pollutants.

• 出版日期2014-9-16
• 单位南京大学