A NOVEL METHOD FOR EFFICIENT GENE DELIVERY USING AMINO-MODIFIED SILICA COATED MAGNETIC NANOPARTICLES

作者：He, X X; Wang, K M^*; Tan, W H; Lin, X; Chen, L; Chen, X H

来源：Reviews on Advanced Materials Science, 2003, 5(4): 375-380.

We have developed a novel method for efficient gene delivery using amino-modified silica coated magnetic nanoparticles. The positively charged amino-modified silica coated magnetic nanoparticles have been directly prepared using the synchronous hydrolysis of tetraethoxysilane and N-(beta-amimoethyl)-gamma-aminopropyltriethoxysilane in water-in-oil microemulsion after the synthesis of aqueous magnetic ferrofluid with the precipitation of the chloride mixture with the base. Plasmid DNA can be easily combined onto the positively charged nanoparticle surface to form Nanoparticles-plasmid DNA complexes. The combined plasmid DNA was effectively protected from enzymatic digestion of DNasel. And the nanoparticles-plasmid DNA complexes can successfully cross various systemic barriers to COS-7 cells as well as mediate high expression of Green Fluorescence Protein (GFP) gene in cells by use of this new amino-modified silica coated magnetic nanoparticles as gene delivery. It would provide a new technology and measure for gene expression, gene filtration, gene function research and gene therapy.

• 出版日期2003
• 单位湖南大学