Extraction and separation of (D,L)-lactic acid in simulated fermentation broth

作者：Zhou Jun; Bi Wentao; Row Kyung Ho^*

来源：Journal of the Indian Chemical Society, 2012, 89(5): 631-636.

• Lactic acid
• fermentation
• extraction
• separation
• ligand exchange

The recovery of lactic acid from fermentation broth plays an important part in production of lactic acid. In this case, the extraction of lactic acid from simulated fermentation broth was processed by tri-n-octylamine dissolved in oleyl alcohol. The extraction efficiency was investigated with several variables, and the optimal condition of extraction of lactic acid (10 mg/mL) from aqueous solution was tri-n-octylamine/oleyl alcohol (30/70, v/v) and solvent phase/fermentation (1/2, v/v) stirring for 60 min under room temperature. The optimal back extraction of lactic acid was obtained by hot water (similar to 90 degrees C) with solvent phase/water (1/4, v/v). The back extracted racemic lactic acid was direct enantiomeric analyzed and separated by chiral ligand chromatography due to strict requirement of absolute configuration in pharmaceutical field and food science. The effect of various parameters on enantioselectivity was discussed and the ((L)-phenylalaninamide center dot HCl (6.0 mmol/L) and CuSO(4 center dot)5H(2)O (3.0 mmol/L) dissolved in methanol/water (5/95, v/v) it pH 6.0 was the suitable mobile phase for chiral ligand exchange separation of (D,L)-lactic acids. By the investigations, a convenient systemic method was established for extraction and separation of (D,L)-lactic acid.

• 出版日期2012-5