Alterations in mineral properties of zebrafish skeletal bone induced by liliput(dtc232) gene mutation

作者：Wang XM; Cui FZ^*; Ge J; Ma C

来源：Journal of Crystal Growth, 2003, 258(3-4): 394-401.

DOI：10.1016/S0022-0248(03)01543-4

• fourier transform infrared microspectroscopy
• mutant
• natural crystal growth
• bone

The alterations of mineral properties of bone by gene mutation in the zebrafish, which is associated with abnormal bone mineralization and bone diseases, were reported for the first time in this paper. Transmission electron microscope (TEM), Fourier transform infrared microspectroscopy (FTIRM). and thermogravimetric analysis (TGA) were used to investigate the changes in the mineral. Significant variations of the morphologies of the minerals, and the mineral/matrix ratio after liliput(dtc232) (lil) gene mutation have been observed. The morphologies of the minerals, examined by TEM, revealed that the mutated mineral was in bigger size and the shape was block shaped but not plate shaped. The results of FTIRM indicated that the lil mutant zebrafish skeleton exhibited a greater mineral/matrix ratio (phosphate/matrix = 4.86 +/- 0.28) than that of wild-type zebrafish bone (phosphate/matrix = 4.17 +/- 0.67), which was confirmed by TGA analysis. Furthermore, the mineral of lil bone became less mature and crystalline with more ion substitutions. And the selected areas electron diffraction (SAED) patterns showed that the main crystal phases of the two type fishes were both hydroxyapatite. In addition, we have discussed the relationship among the mineral properties, nanomechanical properties and biomineralization process.

• 出版日期2003-11
• 单位清华大学