A cascading reaction sequence involving ligand-directed azaelectrocyclization and autooxidation-induced fluorescence recovery enables visualization of target proteins on the surfaces of live cells

作者：Tanaka Katsunori^*; Kitadani Masataka; Tsutsui Ayumi; Pradipta Ambara R; Imamaki Rie; Kitazume Shinobu; Taniguchi Naoyuki; Fukase Koichi

来源：Organic and Biomolecular Chemistry, 2014, 12(9): 1412-1418.

DOI：10.1039/c3ob42267d

A general probe designed to induce a cascading sequence of reactions on a target protein was efficiently synthesized. The cascading reaction sequence involved (i) ligand-directed azaelectrocyclization with lysine and (ii) the autooxidation-induced release of a fluorescence quencher from the labeled protein. The probe was linked to a cyclic RGDyK peptide to enable the selective visualization of integrin alpha(V)beta(3) on the surfaces of live cells.

• 出版日期2014
• 单位RIKEN