Adverse effect of DEHP exposure on the serum insulin level of Balb/c mice

作者：Wang, Jing; Li, Jinquan; Zahid, Kashif Rafiq; Wang, Kun; Qian, Yan; Ma, Ping; Ding, Shumao; Yang, Xu^*; Wang, Xianliang^*

来源：Molecular & Cellular Toxicology, 2016, 12(1): 83-91.

DOI：10.1007/s13273-016-0011-4

• DEHP exposure
• Diabetes-like symptom
• Insulin
• Blood glucose
• Pancreatic cell
• Oxidative stress

Di(2-ethylhexyl) phthalate (DEHP) is a common indoor pollutant in the world, which may cause lots of harmful effects in human including diabetes according to epidemiological studies. To explore the underlying role of DEHP in diabetes-like symptoms, Balb/c mice were chose to be the experimental animals in this paper. They were separated as eight groups as follows: (1) saline + normal diet (vehicle control), (2) 10 mg/kg.day DEHP + normal diet, (3) 50 mg/kg.day DEHP + normal diet, (4) 250 mg/kg.day DEHP + normal diet, (5) streptozotocin (STZ) + high fat diet (diabetes model), (6) 10 mg/kg.day DEHP + STZ + high fat diet, (7) 50 mg/kg.day DEHP + STZ + high fat diet, (8) 250 mg/kg.day DEHP + STZ + high fat diet. The biomarkers of this experiment include four groups: (1) general indicates: body weight and drinking water, (2) blood biomarkers: serum insulin and fasting glucose, (3) pathological examination: pancreas section and kidney section, and (4) biomarkers of oxidative stress: reactive oxygen species (ROS) and malondialdehyde (MDA) in liver cells. Our study results demonstrate that: (1) at our treatment levels DEHP cannot directly induce diabetes, but reduce serum insulin level in DEHP-exposed non-STZ-treated animals, (2) pathological examination finds that these is a dose -dependent damage in the pancreas in DE HP-exposed STZ-treated groups, and (3) the oxidative mechanism may be involved in this pathological process.

• 出版日期2016-3-31
• 单位中国疾病预防控制中心; 华中师范大学; 湖北省疾病预防控制中心; 湖北科技学院