Activation of FoxM1 Revitalizes the Replicative Potential of Aged beta-Cells in Male Mice and Enhances Insulin Secretion

作者：Golson Maria L; Dunn Jennifer C; Maulis Matthew F; Dadi Prasanna K; Osipovich Anna B; Magnuson Mark A; Jacobson David A; Gannon Maureen^*

来源：Diabetes, 2015, 64(11): 3829-3838.

DOI：10.2337/db15-0465

Type 2 diabetes incidence increases with age, while beta-cell replication declines. The transcription factor FoxM1 is required for beta-cell replication in various situations, and its expression declines with age. We hypothesized that increased FoxM1 activity in aged beta-cells would rejuvenate proliferation. Induction of an activated form of FoxM1 was sufficient to increase beta-cell mass and proliferation in 12-month-old male mice after just 2 weeks. Unexpectedly, at 2 months of age, induction of activated FoxM1 in male mice improved glucose homeostasis with unchanged beta-cell mass. Cells expressing activated FoxM1 demonstrated enhanced glucose-stimulated Ca2+ influx, which resulted in improved glucose tolerance through enhanced beta-cell function. Conversely, our laboratory has previously demonstrated that mice lacking FoxM1 in the pancreas display glucose intolerance or diabetes with only a 60% reduction in beta-cell mass, suggesting that the loss of FoxM1 is detrimental to beta-cell function. Ex vivo insulin secretion was therefore examined in size-matched islets from young mice lacking FoxM1 in beta-cells. Foxm1-deficient islets indeed displayed reduced insulin secretion. Our studies reveal that activated FoxM1 increases beta-cell replication while simultaneously enhancing insulin secretion and improving glucose homeostasis, making FoxM1 an attractive therapeutic target for diabetes.

• 出版日期2015-11