An oral vaccine against candidiasis generated by a yeast molecular display system

作者：Shibasaki Seiji^*; Aoki Wataru; Nomura Takashi; Miyoshi Ayuko; Tafuku Senji; Sewaki Tomomitsu; Ueda Mitsuyoshi

来源：Pathogens and Disease, 2013, 69(3): 262-268.

DOI：10.1111/2049-632X.12068

• Eno1p
• Candida albicans
• Saccharomyces cerevisiae
• cell surface engineering

Enolase 1 (Eno1p) of Candida albicans is an immunodominant antigen. However, conventional technologies for preparing an injectable vaccine require purification of the antigenic protein and preparation of an adjuvant. To develop a novel type of oral vaccine against candidiasis, we generated Saccharomyces cerevisiae cells that display the Eno1p antigen on their surfaces. Oral delivery of the engineered S.cerevisiae cells prolonged survival rate of mice that were subsequently challenged with C.albicans. Given that a vaccine produced using molecular display technology avoids the need for protein purification, this oral vaccine offers a promising alternative to the use of conventional and injectable vaccines for preventing a range of infectious diseases. %26lt;br%26gt;This paper deals with the development of an oral vaccine against Enolase 1 (Eno1p) of Candida albicans. The approach taken by using cell surface engineering of Saccharomyces cerevisiae is original and a proper example of how modern oral vaccines can be produced.

• 出版日期2013-12