Application of modified yeast surface display technologies for non-Antibody protein engineering

作者：Mei, Meng; Zhou, Yu; Peng, Wenfang; Yu, Chan; Ma, Lixin; Zhang, Guimin^*; Yi, Li^*

来源：Microbiological Research, 2017, 196: 118-128.

DOI：10.1016/j.micres.2016.12.002

• Yeast surface display
• FACS
• Directed evolution
• Non-antibody protein engineering

Yeast surface display (YSD) system has been widely used in protein engineering since it was established 20 years ago. Combined with fluorescence-activated cell sorting (FACS) technology and directed evolution, YSD has been proven of its extraordinary effectiveness for molecular engineering of various target proteins, especially for antibodies. Recently, a few remarkable efforts were exploited to modify the original Aga1-Aga2 YSD for the non-antibody protein engineering with successful outcomes, expanding its application on oxidase, Class II major histocompatibility complex (MHC-II), protease, sortase, lipoic acid ligase etc. Here, the methodologies of these optimized Aga1-Aga2 YSD technologies were introduced, and the recent progress of non-antibody protein engineering using these methods was summarized.

• 出版日期2017
• 单位湖北大学