MTR and In-vivo H-1-MRS studies on mouse brain with parkinson%26apos;s disease

作者：Yoon Moon Hyun^*; Kim Hyeon Jin; Chung Jin Yeung; Doo Ah Reum; Park Hi Joon; Kim Seung Nam; Choe Bo Young

来源：Journal of the Korean Physical Society, 2012, 61(11): 1852-1859.

DOI：10.3938/jkps.61.1852

• Parkinson%26apos
• s disease (PD)
• Mouse brain
• Magnetization transfer ratio (MTR) histogram parameter
• in vivo H-1 magnetic resonance spectroscopy (H-1-MRS)

The aim of this study was to investigate whether the changes in the magnetization transfer ratio (MTR) histogram are related to specific characteristics of Parkinson%26apos;s disease (PD) and to investigate whether the MTR histogram parameters are associated with neurochemical dysfunction by performing in vivo proton magnetic resonance spectroscopy (H-1-MRS). MTR and in vivo H-1-MRS studies were performed on control mice (n = 10) and 1-methyl-1,2,3,6-tetrahydropyridine intoxicated mice (n = 10). All the MTR and in vivo H-1-MRS experiments were performed on a 9.4 T MRI/MRS system (Bruker Biospin, Germany) using a standard head coil. The protondensity fast spin echo (FSE) images and the T2-weighted spin echo (SE) images were acquired with no gap. Outer volume suppression (OVS), combined with the ultra-short echo-time stimulated echo acquisition mode (STEAM), was used for the localized in-vivo H-1-MRS. The quantitative analysis of metabolites was performed from the H-1 spectra obtained in vivo on the striatum (ST) by using jMRUI (Lyon, France). The peak height of the MTR histograms in the PD model group was significantly lower than that in the control group (p %26lt; 0.05). The midbrain MTR values for volume were lower in the PD group than the control group(p %26lt; 0.05). The complex peak (Glx: glutamine+glutamate+ GABA)/creatine (Cr) ratio of the right ST in the PD group was significantly increased as compared to that of the control group. The present study revealed that the peak height of the MTR histogram was significantly decreased in the ST and substantia nigra, and a significant increase in the Gl (x) /Cr ratio was found in the ST of the PD group, as compared with that of the control group. These findings could reflect the early phase of neuronal dysfunction of neurotransmitters.

• 出版日期2012-12