Branched and linear lipopeptide vaccines have different effects on primary CD4(+) and CD8(+) T-cell activation but induce similar tumor-protective memory CD8(+) T-cell responses

作者：Baz Adriana; Buttigieg Kathy; Zeng Weiguang; Rizkalla Michael; Jackson David C; Groves Penny; Kelso Anne^*

来源：Vaccine, 2008, 26(21): 2570-2579.

DOI：10.1016/j.vaccine.2008.03.022

• lipopeptide vaccines
• T-cell memory
• granzymes
• CTL
• tumor immunity

We compared murine T-cell responses to synthetic lipopeptide vaccines in which the TLR2 ligand Pam(2)Cys was attached to co-linear CD4(+) and CD8(+) T-cell epitopes of ovalbumin (OVA) in a linear or branched configuration. Mice received OVA-specific transgenic CD8(+) and CD4(+) T-cells followed by one injection of vaccine. Although the branched lipopeptide was more potent in activating OVA-specific CD4(+) and CD8(+) T-cells in the primary response, both vaccines induced cytolytic T lymphocytes (CTL) that expressed perforin, granzyme A-C, and IFN-gamma mRNAs and conferred tong-term protection of most mice against challenge with OVA-expressing tumor cells. OVA epitope display was reduced in tumors that developed in some mice, suggesting CD8(+) T-cell dependent selection.

• 出版日期2008-5-19
• 单位河北医科大学