Modular delivery of CpG-incorporated lipid-DNA nanoparticles for spleen DC activation

作者：Jin, Jun-O; Park, Haein; Zhang, Wei; de Vries, Jan Willem; Gruszka, Agnieszka; Lee, Myung Won; Ahn, Dae-Ro; Herrmann, Andreas^*; Kwak, Minseok^*

来源：Biomaterials, 2017, 115: 81-89.

DOI：10.1016/j.biomaterials.2016.11.020

• Dendritic cells
• Immunology
• Adjuvants
• Nanomedicine
• DNA nanotechnology

We introduce a versatile carrier system for in vitro and in vivo immune stimulation based on soft matter DNA nanoparticles (NPs). The incorporation of lipid-modified nucleotides into DNA strands enables the formation of micelles of uniform size. In a single self-assembly step, the micelles can be equipped with immune adjuvant (CpG) motifs and fluorescent probes. The immunological effects of CpG confined at the NP surface were studied in a comprehensive manner in animal experiments. Dose-dependent activation of spleen dendritic cells (DCs) by CpG-conjugated NP was observed, which was accompanied by the pronounced up-regulation of co-stimulatory molecule and cytokine production.

• 出版日期2017-1
• 单位复旦大学