Nonlinear optical microscopy for label-free detection of gastrointestinal neuroendocrine tumors

作者：Li, Lianhuang; Jiang, Liwei; Chen, Zhifen; Kang, Deyong; Yang, Zhenrong; Liu, Xing; Jiang, Weizhong; Zhuo, Shuangmu; Guan, Guoxian; Zhou, Yongjian^*; Chen, Jianxin^*

来源：Lasers in Medical Science, 2016, 31(7): 1285-1291.

DOI：10.1007/s10103-016-1964-1

• Gastrointestinal neuroendocrine tumors
• Nonlinear optical microscopy
• Second harmonic generation (SHG)
• Two-photon excited fluorescence (TPEF)

Neuroendocrine tumors (NETs), which are rare and slow-growing neoplasms, pose a diagnostic challenge as they are clinically silent at the time of presentation. Here, gastrointestinal neuroendocrine tumors were researched by nonlinear microscopy, and results demonstrate that this technique has the capability to identify neuroendocrine tumors in the absence of labels and can, in particular, detect rare neuroendocrine tumor cells, vascular invasion, desmoplastic reaction, and fibroelastosis induced by neuroendocrine tumors. These conclusions highlight the possibility of nonlinear optical microscopy as a diagnostic tool for label-freely differentiating neuroendocrine tumors by these histopathologic features.

• 出版日期2016-9
• 单位福建医科大学; 福建师范大学