科研之友
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摘要
An electrochemical impedance biosensor was proposed for the detection of small molecule biotin based on the plasma-polymerized films (PPF) and the bioaffinity difference between an analyte (biotin) and an analogue compound (HABA) in binding avidin. Avidin formed a metastable complex with 2-[(4-hydroxyphenyl)azo]benzoic acid (HABA) immobilized on the electrode surface. When the sensor contacts a sample solution containing biotin, the avidin was released from the sensor surface to form a more stable complex with biotin in solution. The impedance spectra change recorded is proportional to the desorbed mass of avidin, and there is a clear mathematic relationship between the impedance change and the biotin concentration. The proposed electrochemical impedance bioaffinity sensor has nice response to biotin in the range of 4.8 x 10(-9)-5.6 x 10(-4) M. The sensor could be regenerated under very mild conditions simply by reimmersion of the sensor into a biotin solution to desorb the surplus avidin.
- 出版日期2011
- 单位中国科学院长春应用化学研究所; 中南大学
