Nuclear factor-kappa B is involved in the phenotype loss of parvalbumin-interneurons in vitro

作者：Wang Xian; Zhou Zhiqiang; Yang Chun; Xu Jianguo; Yang Jianjun^*

来源：NeuroReport, 2011, 22(6): 264-268.

DOI：10.1097/WNR.0b013e3283451787

• ketamine
• nuclear factor-kappa B
• oxidative stress
• parvalbumin-containing interneurons
• primary neuronal cultures

The phenotype loss of parvalbumin-containing interneurons, characterized by decreased parvalbumin expression, has been observed in schizophrenic patients. Overproduction of intraneuronal reactive oxygen species leads to such a phenotype loss. Nuclear factor-kappa B (NF-kappa B) activation is both a target and a regulator of intracellular oxidative stress response, suggesting its involvement in the parvalbumin regulation. This study was carried out to investigate the role of the NF-kappa B activation in the ketamine-induced phenotype loss of parvalbumin-interneurons in vitro. Ketamine was applied to primary neuronal cultures to successfully evoke the production of increased reactive oxygen species and decreased parvalbumin expression in parvalbumin-interneurons, which was invalid in the presence of a NF-kappa B inhibitor, SN50 or Bay11-7082. These results suggest potential links among NF-kappa B activation, oxidative stress, and parvalbumin-interneurons in vitro.

• 出版日期2011-4-20
• 单位南京大学