Tomographic imaging of ratiometric fluorescence resonance energy transfer in scattering media

作者：Zhang Yi; Cao Xu; Xu Yanyan; Liu Qing; Zhang Yue; Luo Jianwen; Liu Xiaodong; Bai Jing^*

来源：Applied Optics, 2012, 51(21): 5044-5050.

A method to visualize and quantify fluorescence resonance energy transfer (FRET) in scattering media is proposed. It combines the ratiometric FRET method with fluorescence molecular tomography (FMT) in continuous wave (CW) mode. To evaluate the performance of the proposed method, experiments on a tissue-mimicking phantom are carried out. The results demonstrate that the proposed approach is capable of visualizing and quantifying the FRET distribution in scattering media, which implies the further application of the ratiometric assay in in vivo studies.

• 出版日期2012-7-20
• 单位清华大学