Detection of Helicobacter pylori in the coastal waters of Georgia, Puerto Rico and Trinidad

作者：Holman Chelsea B; Bachoon D S^*; Otero Ernesto; Ramsubhag Adesh

来源：Marine Pollution Bulletin, 2014, 79(1-2): 354-358.

DOI：10.1016/j.marpolbul.2013.11.021

• Helicobacter pylori
• Fecal pollution
• Caribbean
• Pathogens
• Marine pollution

Fecal pollution in the coastal marine environments was assessed at eleven sampling locations along the Georgia coast and Trinidad, and nine sites from Puerto-Rico. Membrane filtration (EPA method 1604 and method 1600) was utilized for Escherichia coil and enterococci enumeration at each location. Quantitative polymerase chain reaction (qPCR) amplification of the 16S ribosomal RNA gene was used to determine the presence of the Helicobacter pylori in marine samples. There was no significant correlation between the levels of E. coil, enterococci and H. pylori in these water samples. H. pylori was detected at four of the 31 locations sampled; Oak Grove Island and Village Creek Landing in Georgia, Maracas river in Trinidad, and Ceiba Creek in Puerto Rico. The study confirms the potential public health risk to humans due to the widespread distribution of H. pylori in subtropical and tropical costal marine waters.

• 出版日期2014-2-15