Anti-Mullerian hormone remains highly expressed in human cumulus cells during the final stages of folliculogenesis

作者：Grondahl M L^*; Nielsen M Eilso; Dal Canto M B; Fadini R; Rasmussen I A; Westergaard L G; Kristensen S G; Andersen C Yding

来源：Reproductive BioMedicine Online, 2011, 22(4): 389-398.

DOI：10.1016/j.rbmo.2010.12.005

• AMH
• AMHR2
• CYP19
• human large antral follicles
• steroids

This study evaluated whether anti-Mullerian hormone (AMH) was differentially expressed in cumulus (CC) and granulosa (GC) cells from large antral and pre-ovulatory follicles collected from individual follicles in women undergoing in-vitro maturation (IVM) or IVF treatment. Expression studies of AMH, AMH receptor 2, FSH receptor, aromatase and androgen receptor were performed in CC in IVM patients where cumulus-oocyte-complex had expanded, CC in IVM patients where cumulus-oocyte-complex remained compacted, GC from immature follicles and CC and GC from IVF patients. Microarray data on corresponding GC and CC from follicles from IVF patients was included. AMH expression was significantly higher in CC than in GC from both mature and immature follicles and in CC from immature follicles than in CC from pre-ovulatory follicles from IVF patients (P < 0.05). AMH expression was significantly higher in CC that remained compacted compared with those that had expanded (P < 0.008). AMH was correlated to the expression of FSH receptor, androgen receptor and AMH receptor 2 but not to aromatase expression. The expression pattern of AMH receptor 2 reflected that of AMH. AMH may exert intrafollicular functions even in human large antral and pre-ovulatory follicles and may be related to follicular health.

• 出版日期2011-4