Protein design in biological networks: from manipulating the input to modifying the output

作者：Van der Sloot Almer M^*; Kiel Christina; Serrano Luis; Stricher Francois

来源：Protein Engineering Design and Selection, 2009, 22(9): 537-542.

DOI：10.1093/protein/gzp032

• protein design
• protein interaction networks
• signaling
• synthetic biology
• systems biology

Protein engineering has been an invaluable tool for the deciphering of protein folding and function and in the understanding of biological signaling networks. From an applied point of view it has been of paramount importance in biotechnological and biopharmaceutical products and applications. Traditionally, the protein engineering tools of choice were 'classical' rational design, or directed evolution-based methods. In recent years, a third tool has matured: computational protein design (CPD). In this review, we summarize the underlying principles of CPD and discuss its application for understanding and modifying biological systems. Three main applications of the use of protein design will be highlighted and reviewed: artificially rewiring of signal transduction networks, prediction and generation of large-scale in silico interaction networks and using protein design to manipulate gene expression.

• 出版日期2009-9