NMR as Evaluation Strategy for Cellular Uptake of Nanoparticles

作者：Orlando Tomas^*; Paolini Alessandro; Pineider Francesco; Clementi Emanuela; Pasi Francesca; Guari Yannick; Larionova Joulia; Sacchi Luciano; Nano Rosanna; Corti Maurizio; Lascialfari Alessandro

来源：Nano Letters, 2014, 14(7): 3959-3965.

DOI：10.1021/nl501282x

• Magnetic nanoparticles
• gold nanoparticles
• cellular uptake
• H-1 NMR
• cellular response

Advanced nanostructured materials, such as gold nanoparticles, magnetic nanoparticles, and multifunctional materials, are nowadays used in many state-of-the-art biomedical application. However, although the engineering in this field is very advanced, there remain some fundamental problems involving the interaction mechanisms between nanostructures and cells or tissues. Here we show the potential of H-1 NMR in the investigation of the uptake of two different kinds of nanostructures, that is, maghemite and gold nanoparticles, and of a chemotherapy drug (Temozolomide) in glioblastoma tumor cells. The proposed experimental protocol provides a new way to investigate the general problem of cellular uptake for a variety of biocompatible nanostructures and drugs.

• 出版日期2014-7