Four-photon-excited fluorescence resonance energy transfer in an aqueous system from ZnSe: Mn/ZnS quantum dots to hypocrellin A

作者：Feng, Yueshu; Liu, Liwei^*; Hu, Siyi; Ren, Yu; Liu, Yingyi; Xiu, Jingrui; Zhang, Xihe

来源：Optics Express, 2016, 24(17): 19627-19637.

DOI：10.1364/OE.24.019627

• PHOTODYNAMIC THERAPY
• FRET
• 2-PHOTON
• EXCITATION
• CONVERSION
• VIVO
• NANOCRYSTALS
• LUMINESCENCE
• EMISSION

In this work, we established a fluorescence resonance energy transfer (FRET) system between ZnSe: Mn/ZnS quantum dots and Hypocrellin A (HA, a photosensitizer used for photodynamic therapy of cancer) in aqueous solution, excited by four-photon. Here, the QDs are the donors and the HA are the acceptors. The four-photon-excited fluorescence resonance energy transfer spectrum was obtained under 1300nm femtosecond laser pluses. The experimental results indicated that the highest efficiency of FRET can reach up to 61.3%. Furthermore, the viability test in cancer cells was further demonstrated for biological applications of FRET system. When FRET occurs the cell killing rate of the cancer cells will reach to 84.8% with the 1mM concentration of HA. Our work demonstrates that while the four-photon excited FRET system is promising in both optics and biological applications, is also needs further investigation.

• 出版日期2016-8-22
• 单位长春理工大学