Anti-mitotic potential of 7-diethylamino-3(2 %26apos;-benzoxazolyl)-coumarin in 5-fluorouracil-resistant human gastric cancer cell line SNU620/5-FU

作者：Kim Nam Hyun; Kim Su Nam; Oh Joa Sub; Lee Seokjoon; Kim Yong Kee^*

来源：Biochemical and Biophysical Research Communications, 2012, 418(4): 616-621.

DOI：10.1016/j.bbrc.2012.01.049

• 7-Diethylamino-3(2 %26apos
• -benzoxazolyl)-coumarin
• Microtubule
• Drug resistance
• 5FU

In this study, we investigate an anti-mitotic potential of the novel synthetic coumarin-based compound, 7-diethylamino-3(2%26apos;-benzoxazolyl)-coumarin, in 5-fluorouracil-resistant human gastric cancer cell line SNU-620-5FU and its parental cell SNU-620. It exerts the anti-proliferative effects with similar potencies against both cancer cells, which is mediated by destabilization of microtubules and subsequent mitotic arrest. Furthermore, this compound enhances caspase-dependent apoptotic cell death via decreased expression of anti-apoptotic genes. Taken together, our data strongly support anti-mitotic potential of 7-diethylamino-3(2%26apos;-benzoxazolyl)-coumarin against drug-resistant cancer cells which will prompt us to further develop as a novel microtubule inhibitor for drug-resistant cancer chemotherapy.

• 出版日期2012-2-24