Assessing and screening for T-cell epitopes from Mycobacterium tuberculosis RD2 proteins for the diagnosis of active tuberculosis

作者：Jiazhen Chen; Qiaoling Ruan; Yaojie Shen; Sen Wang; Lingyun Shao; Wenhong Zhang

来源：Brazilian Journal of Infectious Diseases, 2018, 22(6): 462-471.

DOI：10.1016/j.bjid.2018.10.280

• Mycobacterium tuberculosis
• T-cell epitope
• Diagnostic antigen

Abstract(#br)The Region of D eletion 2 (RD2) of Mycobacterium tuberculosis encodes reserved antigens that contribute to bacterial virulence. Among these antigens, Rv1983, Rv1986, Rv1987, and Rv1989c have been shown to be immunodominant in infected cattle; however, their diagnostic utility has not been evaluated in humans.(#br)In this study, we screened 87 overlapping synthetic peptides encoded by five RD2 proteins for diagnosing tuberculosis epitopes in 50 active tuberculosis (TB) cases, 31 non-tuberculosis patients and 36 healthy individuals. A pool of promising epitopes was then assessed for their diagnostic value in 233 suspected TB patients using a whole blood IFN-γ release assay.(#br)Only 10 peptides were recognized by more than 10% of active tuberculosis patients. The IFN-γ release responses to Rv1986-P9, P15, P16, Rv1988-P4, P11, and Rv1987-P11 were significantly higher in the active TB group than in the control groups ( p <ce:hsp sp="0.25"/><<ce:hsp sp="0.25"/>0.05). The whole blood IFN-γ release assay based on these epitopes yielded a sensitivity of 51% and a specificity of 85% in diagnosing active tuberculosis, and the corresponding results using the T-SPOT. TB assay were 76% and 75%, respectively.(#br)In conclusion, these results suggest that the six epitopes from the RD2 of M. tuberculosis have potential diagnostic value in TB.

• 出版日期2018
• 单位复旦大学