Apoptotic Effects of Protocatechuic Acid in Human Breast, Lung, Liver, Cervix, and Prostate Cancer Cells: Potential Mechanisms of Action

作者：Yin Mei Chin^*; Lin Chun Che; Wu Hsi Chin; Tsao Shih Ming; Hsu Cheng Kuang

来源：Journal of Agricultural and Food Chemistry, 2009, 57(14): 6468-6473.

DOI：10.1021/jf9004466

• Protocatechuic acid
• cancer cells
• Na(+)-K(+)-ATPase activity
• caspase activity
• DNA fragmentation

Apoptotic effects of protocatechuic acid (PCA) at 1, 2, 4, 8 mu mol/L on human breast cancer MCF7 cell, lung cancer A549 cell, HepG2 cell, cervix HeLa cell, and prostate cancer LNCaP cell were examined. Results showed that PCA concentration-dependently decreased cell viability, increased lactate dehydrogenase leakage, enhanced DNA fragmentation, reduced mitochondrial membrane potential, and lowered Na(+)-K(+)-ATPase activity for these cancer cells (P < 0.05). PCA also concentration-dependently elevated caspase-3 activity in five cancer cells (P<0.05), but this agent at 2-8 mu mol/L significantly increased caspase-8 activity (P<0.05). PCA concentration-dependently decreased intercellular adhesion molecule level in test cancer cells (P < 0.05) but significantly inhibited cell adhesion at 2-8 mu mol/L (P < 0.05). PCA also concentration-dependently lowered the levels of interleukin (IL)-6 and IL-8 in five cancer cells (P < 0.05), but this agent at 2-8 mu mol/L significantly suppressed vascular endothelial growth factor production (P < 0.05). These findings suggest that PCA is a potent anticancer agent to cause apoptosis or retard invasion and metastasis in these five cancer cells.

• 出版日期2009-7-22
• 单位中国医科大学