Diterpene Alkaloids from Aconitum anthora and Assessment of the hERG-Inhibiting Ability of Aconitum Alkaloids

作者：Forgo Peter; Borcsa Botond; Csupor Dezso; Fodor Laszlo; Robert Berkecz; Molnar Attila V; Hohmann Judit^*

来源：Planta Medica, 2011, 77(4): 368-373.

DOI：10.1055/s-0030-1250362

• Aconitum anthora
• Ranunculaceae
• aconitine
• diterpene alkaloids
• hERG
• potassium channels

A new norditerpene alkaloid, 10-hydroxy-8-O-methyltalatizamine (1), was isolated from the whole plant of Aconitum anthora L. besides the known isotalatizidine (2) and hetisinone (3). The structures were determined by means of HR-ESI-MS, 1D and 2D NMR spectroscopy, including (1)H-(1)H COSY, NOESY, HSQC and HMBC experiments, resulting in complete (1)H and (13)C chemical shift assignments for 1-3, and revision of some earlier (13)C-NMR data. The effects of the isolated compounds, together with twenty-one other Aconitum alkaloids with different skeletal types and substitution patterns, on hERG channels were studied by the whole-cell patch clamp technique, using the QPatch-16 automated patch clamp system. At 10 mu M, aconitine, 14-benzoylaconine 8-O-palmitate, songoramine, gigactonine and neolinine demonstrated significant hERG K(+) channel inhibition; all other compounds exerted only low (6-21%) inhibitory activity.

• 出版日期2011-3