Acanthamoeba genotypes T2, T4, and T11 in soil sources from El Hierro island, Canary Islands, Spain

作者：Reyes Batlle Maria; Zamora Herrera Jonadab; Vargas Mesa Alejandro; Antonio Valeron Tejera Marco; Wagner Carolina; Ma Martin Navarro Carmen; Lopez Arencibia Atteneri; Sifaoui Ines; Martinez Carretero Enrique; Valladares Basilio; Pinero Jose E; Lorenzo Morales Jacob^*

来源：Parasitology Research, 2016, 115(8): 2953-2956.

DOI：10.1007/s00436-016-5048-8

• Acanthamoeba
• El Hierro
• Genotype
• Canary Islands
• Soil

The genus Acanthamoeba includes pathogenic strains which are causative agents of keratitis and encephalitis that often may end fatal in humans and other animals. In the present study, forty soil samples were collected in the island of El Hierro, Canary Islands, Spain, and checked for the presence of Acanthamoeba. Samples were cultivated onto 2 % non-nutrient agar plates seeded with a layer of heat killed Escherichia coli. Amplification by PCR and sequencing of the DF3 region of the 18S rDNA of Acanthamoeba was carried out in order to confirm morphological identification of the amoebae. Furthermore, Acanthamoeba spp. was isolated from 47.5 % of soil samples. Moreover, genotypes T2, T4, and T11 were identified in these samples. To the best of our knowledge, this is the first study to establish genotypes T2, T4, and T11 in soil sources from El Hierro island.

• 出版日期2016-8