An oil-in-water nanoemulsion enhances immunogenicity of H5N1 vaccine in mice

作者：Cao Weiping^*; Davis William G; Kim Jin Hyang; De La Cruz Juan A; Taylor Andrew; Hendrickson Grant R; Kumar Amrita; Ranjan Priya; Lyon L Andrew; Katz Jacqueline M; Gangappa Shivaprakash; Sambhara Suryaprakash

来源：Nanomedicine: Nanotechnology, Biology and Medicine , 2016, 12(7): 1909-1917.

DOI：10.1016/j.nano.2016.04.005

• Oil-in-water nanoemulsion
• Adjuvant
• H5N1 vaccine
• Innate immunity
• Adaptive immunity
• Influenza

To enhance the immunogenicity of the Influenza H5N1 vaccine, we developed an oil-in-water nanoemulsion ( NE) adjuvant. NE displayed good temperature stability and maintained particle size. More importantly, it significantly enhanced IL-6 and MCP-1 production to recruit innate cells, including neutrophils, monocytes/macrophages and dendritic cells to the local environment. Furthermore, NE enhanced dendritic cell function to induce robust antigen-specific T and B cell immune responses. NE-adjuvanted H5N1 vaccine not only elicited significantly higher and long-lasting antibody responses, but also conferred enhanced protection against homologous clade 1 as well as heterologous clade 2 H5N1 virus challenge in young as well as in aged mice. The pre-existing immunity to seasonal influenza did not affect the immunogenicity of NE-adjuvanted H5N1 vaccine. Published by Elsevier Inc.

• 出版日期2016-10