Microfluidic paper-based device for colorimetric determination of glucose based on a metal-organic framework acting as peroxidase mimetic

作者：Ortiz Gomez Inmaculada; Salinas Castillo Alfonso; Garcia Garcia Amalia; Antonio Alvarez Bermejo Jose; de Orbe Paya Ignacio; Rodriguez Dieguez Antonio; Fermin Capitan Vallvey Luis

来源：Microchimica Acta, 2018, 185(1): UNSP 47.

DOI：10.1007/s00604-017-2575-7

• mu PADs
• Enzyme mimic
• Photographic camera
• Smartphone app
• Colorimetric assay
• Glucose monitoring
• 3,3',5,5'-Tetramethylbenzidine

This work presents a microfluidic paper-based analytical device (mu PAD) for glucose determination using a supported metalorganic framework (MOF) acting as a peroxidase mimic. The catalytic action of glucose oxidase (GOx) on glucose causes the formation of H2O2, and the MOF causes the oxidation of 3,3', 5,5'-tetramethylbenzidine (TMB) by H2O2 to form a blue-green product with an absorption peak at 650 nm in the detection zone. A digital camera and the iOS feature of a smartphone are used for the quantitation of glucose with the S coordinate of the HSV color space as the analytical parameter. Different factors such as the concentration of TMB, GOx and MOF, pH and buffer, sample volume, reaction time and reagent position in the mu PAD were optimized. Under optimal conditions, the value for the S coordinate increases linearly up to 150 mu mol.L-1 glucose concentrations, with a 2.5 mu mol.L-1 detection limit. The mu PAD remains stable for 21 days under conventional storage conditions. Such an enzyme mimetic-based assay to glucose determination using Fe-MIL-101 MOF implemented in a microfluidic paper-based device possesses advantages over enzyme-based assays in terms of costs, durability and stability compared to other existing glucose determination methods. The procedure was applied to the determination of glucose in (spiked) serum and urine.

• 出版日期2018-1