Glypican-3 expression and its relationship with recurrence of HCC after liver transplantation

作者：Wang, Yu-Liang; Zhu, Zhi-Jun; Teng, Da-Hong; Yao, Zhi; Gao, Wei; Shen, Zhong-Yang^*

来源：World Journal of Gastroenterology, 2012, 18(19): 2408-2414.

DOI：10.3748/wjg.v18.i19.2408

• Liver transplantation
• Hepatocellular carcinoma
• Glypican-3
• mRNA
• Recurrence

AIM: To investigate the diagnostic value of glypican-3 (GPC3) and its relationship with hepatocellular carcinoma (HCC) recurrence after liver transplantation. @@@ METHODS: HCC tissue samples (n = 31) obtained from patients who had undergone liver transplantation were analyzed. GPC3 mRNA and protein expression were analyzed by TaqMan real-time reverse transcription-polymerase chain reaction and immunohistochemistry. Correlation between the GPC3 expression and clinicopathological features was analyzed. The potential prognostic value of GPC3 was investigated by comparing recurrence-free survival between HCC patients with and without GPC3 expression. @@@ RESULTS: Using a cutoff value of 3.5 x 10(-2), 20 of 31 cancerous tissues had expression values of > 3.5 x 10(-2), whereas 3 of 31 adjacent non-neoplastic parenchyma and 0 of 20 control liver tissues had expression values of > 3.5 x 10(-2) (P < 0.001). GPC3 protein was innmunoexpressed in 68% of cancerous tissues, but not in adjacent non-neoplastic parenchyma and control liver tissues. Vascular invasion was significantly related to GPC3 expression (P < 0.05). Recurrence-free survival was significantly longer for patients without GPC3 mRNA overexpression (> 3.5 x 10(-2)) and those without vascular invasion (P < 0.05 for both). @@@ CONCLUSION: GPC3 expression may serve as a valuable diagnostic marker for HCC. GPC3 mRNA overexpression may be an adverse indicator for HCC patients after liver transplantation.

• 出版日期2012-5-21
• 单位天津市第一中心医院; 天津医科大学