Preparation and Characterization of Water-in-Oil-in-Water Emulsions Containing a High Concentration of L-Ascorbic Acid

作者：Khalid Nauman; Kobayashi Isao^*; Neves Marcos A; Uemura Kunihiko; Nakajima Mitsutoshi

来源：Bioscience Biotechnology and Biochemistry, 2013, 77(6): 1171-1178.

DOI：10.1271/bbb.120870

• W/O/W emulsions
• L-ascorbic acid
• high concentration
• stability
• microstructure

This study sought to encapsulate a high concentration of L-ascorbic acid, up to 30% (w/v), in the inner aqueous phase of water-in-oil-water (W/O/W) emulsions with soybean oil as the oil phase. Two-step homogenization was conducted to prepare W/O/W emulsions stabilized by a hydrophobic emulsifier and 30% (v/v) of W/O droplets stabilized by a hydrophilic emulsifier. First-step homogenization prepared W/O emulsions with an average aqueous droplet diameter of 2.0 to 3.0 mu m. Second-step homogenization prepared W/O/W emulsions with an average W/O droplet diameter of 14 to 18 mu m and coefficients of variation (CVs) of 18% to 25%. The results indicated that stable W/O/W emulsions containing a high concentration of L-ascorbic acid were obtained by adding gelatin and magnesium sulfate in the inner aqueous phase and glucose in both aqueous phases. L-Ascorbic acid retention in the W/O/W emulsions was 40% on day 30 and followed first-order kinetics.

• 出版日期2013-6