Acetaminophen Induces the Transcription of the Antioxidant Proteins Thioredoxin 1 and Glutaredoxin 1 in The Brain and Liver of Balb/C Mice

作者：Limon Pacheco Jorge H; Valdovinos Flores Cesar; Navarrete Leon Roxana M; Gonsebatt Maria E^*

来源：Latin American Journal of Pharmacy, 2016, 35(9): 2029-2035.

• acetaminophen
• antioxidant
• glutaredoxin 1
• thioredoxin 1

Acetaminophen (APAP) is an analgesic and antipyretic drug that can cause severe liver damage when-high doses are-administered. The damage is caused by the depletion of intracellular glutathione (GSH) and an-excess of-the N-acetyl-p-benzoquinone imine metabolite that reacts with cellular and mitochondrial proteins producing cell damage and tissue necrosis. In this study, we analyzed the response to an intraperitoneal injection (300 mg/kg) of APAP at 1, 2 and 3 h after administration in the livers, brains, and kidneys of BALB/c male mice. We observed a significant decrease in the levels of GSH in the liver and kidneys, and an increase in the transcription of Nrf2 and Grx in the liver. In the brain, we found an increase in the transcription of Grx and Trx associated with NF-kappa B nuclear migration. In the kidneys, the up-regulation of these antioxidant proteins was not observed, which coincides with the significant increase in lipid peroxidation.

• 出版日期2016-11