Utility of peripheral blood for cytogenetic and mutation analysis in myelodysplastic syndrome

作者：Mohamedali Azim M; Alkhatabi Heba; Kulasekararaj Austin; Shinde Sneha; Mian Syed; Malik Farooq; Smith Alexander E; Gaeken Joop; Mufti Ghulam J^*

来源：Blood, 2013, 122(4): 567-570.

DOI：10.1182/blood-2012-12-471847

Recent studies have shown that more than 80% of bone marrow (BM) samples from patients with myelodysplastic syndrome (MDS) harbor somatic mutations and/or genomic aberrations, which are of diagnostic and prognostic importance. We investigated the potential use of peripheral blood (PB) and serum to identify and monitor BM-derived genetic markers using high-resolution single nucleotide polymorphism array (SNP-A) karyotyping and parallel sequencing of 22 genes frequently mutated in MDS. This pilot study showed a 100% SNP-A karyotype concordance and a 97% mutation concordance between the BM and PB. In contrast, mutation analysis using Sanger sequencing of PB and serum-derived DNA showed only 65% and 42% concordance to BM, respectively. Our results show the potential utility of PB as a surrogate for BM for MDS patients, thus avoiding the need for repeated BM aspirates particularly in elderly patients and those with fibrotic or hypocellular marrows.

• 出版日期2013-7-25