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摘要
The shelf life of poultry legs stored aerobically and the possible role of the aminopeptidase activity of gram-negative bacteria (p-nitroaniline test) as a predictor of poultry spoilage were evaluated on the basis of microbiological and sensory parameters. Chicken legs (n = 30) obtained immediately after evisceration in a local Poultry processing plant were kept Under aerobic refrigeration (4 +/- 1 degrees C) for 7 days. Microbiological (counts of aerobic bacteria and psychrotrophs) and sensory (odor, color, and general acceptability oil a hedonic Scale of 1 to 9) parameters and aminopeptidase activity (absorbance at 390 nm [A(390)]) determinations were performed after 0, 1, 3, 5, and 7 days of storage. Aerobic plate Counts of 7 log CFU/g and a score of 6 for general acceptability Were used Lis indicators of the end point of shelf life. Strong correlations (r >= 0.76; P < 0.001) were obtained between bacterial counts, hedonic scores, and A(390) values. Samples were judged as unacceptable (shelf-fife end point) after 2 and 4 days on the basis of sensory and microbiological analyses, respectively. A(390) Values of 0.52 and 0.89 (corresponding to p-nitroaniline, concentrations of 6.25 and 10.7 mu g/ml, respectively) are proposed its the upper limits for acceptability on the basis of sensory and microbiological determinations, respectively. However. these recommendations are based on a small set of samples. and their general application is yet to be verified.
- 出版日期2010-2
